SmaI

Brand	Takara Bio

SmaI restriction site:

CCC | GGG
GGG | CCC

Supplied buffer: T+BSA

Effect of DNA methylation: enzyme activity is affected by CG methylase

Reaction temperature: 30°C

Source: Serratia marcescens Sb
Concentration: 4–12 units/µl [High concentration: 30–60 units/µl]
Substrate for unit definition: lambda DNA
Genomic DNA analysis: Staphylococcus aureus genomic DNA
This enzyme shows equivalent activity at 37°C and 30°C, but stability of the enzyme in reaction mixtures is lower at 37°C than at 30°C.
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning