Cloning Enhancer removes background plasmid DNA and PCR residue, eliminating the need for PCR insert purification prior to cloning. Cloning Enhancer-treated PCR samples yield significantly more recombinant clones. Since treatment with Cloning Enhancer occurs in the same tube as the PCR reaction, it is less likely to result in UV damage or nicking. Avoiding a separate cleanup process also minimizes the risk of losing PCR products during purification.
Overview
- Convenient treatment of PCR products without purification
- Single-tube reaction with no sample loss
- Gentle treatment—no DNA damage or nicking
- 5X increase in colony yield compared to unpurified inserts
- Optimized to work with the In-Fusion HD Cloning Plus products
