Y187 Yeast Strain

Additional information

Brand

Size

0.5 mL

The Saccharomyces cerevisiae strain Y187 is for use with Clontech’s Matchmaker Gold Yeast Two-Hybrid System as a mating partner for the Y2HGold reporter strain. Clontech’s Mate & Plate Libraries are constructed in Y187.

Overview

  • Y2HGold provides four reporters for exceptionally stringent two-hybrid screening with Matchmaker Gold
  • Use Y187 for simplified library construction & screening with the Mate & Plate system
  • Y2HGold and Y187 have opposite mating types, so they are perfect mating partners

Applications

  • Yeast two-hybrid screening
  • Yeast two-hybrid library construction

Library generation using n vivo recombination in S. cerevisiae. Mate & Plate Libraries are created via recombination between your cDNA and the Matchmaker prey vector pGADT7-Rec.

 Library generation using n vivo recombination in S. cerevisiae. Mate & Plate Libraries are created via recombination between your cDNA and the Matchmaker prey vector pGADT7-Rec.

Library generation using in vivo recombination in S. cerevisiae. Mate & Plate Libraries are created via recombination between your cDNA and the Matchmaker prey vector pGADT7-Rec. The homologues are generated by SMART cDNA synthesis. Colonies are pooled, mixed, and aliquoted into multiple vials. Each single 1 ml vial can be used for a two-hybrid screen.