XhoI

Brand	Takara Bio

XhoI restriction site:

C | TCGA G
G AGCT | C

Supplied buffer: H

Effect of DNA methylation: enzyme activity is affected by CG methylase (It cleaves CT5m CGAG very slowly)

Reaction temperature: 37°C

Source: Xanthomonas holcicola
Concentration: 4–12 units/µl [High concentration: 30–60 units/µl]
Substrate for unit definition: lambda DNA
Genomic DNA analysis: Escherichia coli genomic DNA
Ligation-recutting test: The ligation efficiency of cohesive end DNA fragments generated by this enzyme will be low. More efficient ligation can be achieved using reaction conditions for blunt end ligation
Each lot undergoes stringent quality testing including: overdigestion, genomic DNA analysis, ligation-recutting, and pKF3 cloning