X-Gal

X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galactoside)

X-Gal (5-Bromo-4-Chloro-3-Indolyl-beta-D-Galactoside) is a chromogenic substrate for β-galactosidase that yields a blue precipitate upon hydrolysis.

Overview

• A histochemical substrate for beta-galactosidase that yields a blue precipitate upon hydrolysis
• Used with IPTG (9030) in media to select recombinants using blue/white colony screening