WST-1 Cell Proliferation Assay Kit

Cell proliferation measurements are proportional to the concentration of the proliferation promoter IL-2

Cell proliferation measurements are proportional to the concentration of the proliferation promoter IL-2. Mouse CTLL-2 T cells were seeded in 96-well plates at 4 x 103 cells/well, with 100 µl culture medium. Increasing concentrations of human IL-2 were added, and the cells were incubated for 48 hr at 37°C with 5 CO2. 10 µl/well of Premixed WST-1 Cell Proliferation Reagent was added, and the cells were incubated for an additional 4 hr under the same conditions. Absorbance at 450 nm was measured in a multiwell plate reader.

Using the Premix WST-1 Cell Proliferation Assay to measure the cytotoxic effects of human tumor necrosis factor (TNF-alpha) on the mouse fibrosarcoma cell line WEHI-164

Using the Premix WST-1 Cell Proliferation Assay to measure the cytotoxic effects of human tumor necrosis factor (TNF-alpha) on the mouse fibrosarcoma cell line WEHI-164. WEHI-164 cells were seeded in 96-well plates at 5 x 104 cells/well in 100 μl culture medium containing increasing concentrations of TNF-alpha. The cells were incubated at 37˚C for 24 hr in a humidified atmosphere maintained at 5 CO2. 10 µl/well of Premixed WST-1 Cell Proliferation Reagent was added, and the cells were incubated for an additional 4 hr under the same conditions. Absorbance at 450 nm was measured in a multiwell plate reader.

Simple WST-1 cell-proliferation protocol

Simple WST-1 cell-proliferation protocol. Know if your cells are proliferating in under an hour.

Cleavage of the tetrazolium salt (WST-1) to formazan

Cleavage of the tetrazolium salt (WST-1) to formazan.