CellAmp Whole Transcriptome Amplification Kit
The CellAmp Whole Transcriptome Amplification Kit (Real Time) Ver. 2 allows direct amplification of cDNA from a small number of cells. The amplified cDNA can be used as a template for real-time PCR. When using conventional techniques for working with trace amounts of nucleic acids, loss of nucleic acid during purification steps is a frequent problem. However, this kit provides good yield because loss-inducing purification steps for RNA and cDNA are not required.
Overview
- Allow direct amplification of cDNA from a small number of cultured cells (<1,000) using real-time RT-PCR
- Allows real-time PCR (qPCR) analysis of multiple genes from small samples
- Expression analysis for low-expressed gene
Applications
- Cells to cDNA with minimal purification
- Real-time RT-PCR (RT-qPCR)
- cDNA amplification from trace amounts of RNA
