Tris-glycine-SDS (TG-SDS) buffer is used for SDS polyacrylamide gel electrophoresis (SDS-PAGE) of protein. These products are powders for preparing Tris-Glycine-SDS buffer.
Applications
- Migration buffer for SDS polyacrylamide gel electrophoresis of protein.
Components
Tris-Glycine-SDS Buffer (TG-SDS) Powder, pH 8.3 (Cat. #T9101), 10 pouches (for 10 L)
Final solution from one pouch (1 L) contains:
- 0.025 M Tris
- 0.192 M Glycine
- 0.10% SDS
Tris-Glycine-SDS Powder (Cat. #T901), 50 packages (for 25 L)
One package (for 500 ml) contains:
- 3.0 g Tris (hydroxymethyl) aminomethane
- 14.4 g Glycine
- 0.5 g SDS
Storage
Store powder at room temperature.
Note
Tris-Glycine-SDS Powder (Cat. #T901) is manufactured by Roman Industries Co., Ltd.
Preparation
Transfer the contents of the pouch to a flask or beaker. Add 300 ml of distilled water or deionized water and stir the solution for a few minutes. Adjust the volume to 1,000 ml, and stir until completely dissolved.
