Tris buffered saline (TBS) buffer is commonly used as a wash and/or dilution solution in a variety of applications including cell culture, Western blotting, ELISA, and immunohistochemistry. Takara Bio offers both tablets and powder for easy preparation of TBS buffer. Tablets are available for TBS and TBS containing Tween 20 (TBS-T).
Applications
- TBS: wash buffer for ELISA, Western blotting, and immunohistochemical staining; sample dilution buffer
- TBS-T: antibody dilution, wash buffer for Western blots and ELISAs
Components
- Tris Buffered Saline (TBS) Tablets, pH 7.6 (Cat. #T9141)
100 tablets (for 50 L)
- Tris Buffered Saline with Tween 20 (TBS-T) Tablets, pH 7.6 (Cat. #T9142)
100 tablets (for 50 L; final solution contains 0.05% Tween 20)
- TBS (Tris-Buffered-Saline) Powder (Cat. #T903)
30 packages (for 30 L)
Storage
Store at room temperature.
Note
TBS (Tris-Buffered-Saline) Powder (Cat. #T903) is manufactured by Roman Industries Co., Ltd.
Preparation
Transfer the contents of the pouch to a flask or beaker. Add 300 ml of distilled water or deionized water and stir the solution for a few minutes. Adjust the volume to 1,000 ml, and stir until completely dissolved.Additional product information
