TransFactor Chemiluminescent Kits provide rapid, sensitive detection of specific transcription factor DNA binding in whole cell or nuclear extracts using an ELISA (enzyme-linked immunosorbent assay) format. Each NFATc1 Chemiluminescent Kit contains two 96-well plates with the consensus binding sequence for NFATc1 coated in the wells. Primary and Secondary Antibodies are provided to detect the bound transcription factor. Competitor Oligos are provided for competition assays.
Overview
- Highly sensitive detection of DNA-transcription factor interactions
- Faster and more sensitive than gel-shift assays
- Nonradioactive, chemiluminescent detection in a flexible, high-throughput format
- Compatible with nuclear, whole-cell, and cytosolic extracts
Applications
- Monitor signal transduction
- Perform multiple experiments in 3–4 hours, without the need for radioactive labeling
- Study DNA-NFATc1 transcription factor interactions
- Examine NFATc1 behavior in a cell line that has been treated with different stimuli
- Compare the relative amounts of the transcription factor NFATc1 in different cell types
