The Titanium One-Step RT-PCR Kit is designed for the reverse transcription of total or poly A+ RNA and the subsequent PCR amplification of a target cDNA in a single reaction tube. The included enzyme mix contains a highly purified Moloney murine leukemia virus (MMLV) reverse transcriptase for first-strand cDNA synthesis, and Titanium Taq DNA Polymerase for PCR amplification. Titanium Taq DNA Polymerase is a blend of a 5′-exonuclease deficient, thermostable DNA polymerase, and TaqStart Antibody for automatic hot start PCR. Reagents for purifying RNA are not supplied.
Overview
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Allows qualitative analysis of gene expression from multiple samples
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Produces results from as little as 10 pg of total RNA
- Provided in a convenient single-tube format
- Easy-to-use one-step format minimizes the risk of cross contamination
Applications
- One-step RT-PCR
- Endpoint RT-PCR
- Gene expression analysis
- First-strand cDNA synthesis
