Antibody purification resins—an alternative to Protein A
Thiophilic-Superflow Resin is an adsorbent for large-scale or FPLC purifcation of various proteins, including IgG, IgM, IgY, and IgE antibodies. Superflow allows for flow rates of as high as 5 ml/cm2 min.
Overview
Thiophilic-Superflow Resin utilizes thiophilic adsorption chromatography. With this technique, protein adsorbs to a sulfone thioether ligand. The adsorption of different proteins can be promoted by adding different salts to the mixture. Varying the concentration of the loading salt can affect the adsorption affinities of IgG, IgM, IgA, Fab and Fc fragments, and C3 and C4 complement factors.
Purification with Thiophilic-Superflow Resin offers several advantages over purification with Protein A, the conventional method used for immunoglobulin purification:
- Purification at neutral pH
- Broader selectivity (IgY, IgM, IgE, scAb)
- High linear flow rates
- High capacity (15–20 mg Ab/ml resin)
- Reusability
- Higher stability of the purified product
Applications
- Antibody purification, including IgG, IgM, and chicken IgY
