Tet-On 3G Transactivator Lentiviral Particles

Various cell types can be transduced with Vectalys lentiviral particles, including primary and stem cell lines

Various cell types can be transduced with Vectalys lentiviral particles, including primary and stem cell lines. The suggested seeding concentration and MOI vary depending on the specific cell type to be transduced. When the recommendations in this table are followed, transduction efficiency is high for immortalized, primary, and stem cells, regardless of whether they are adherent or suspension cells.

Overview of the process for generating high-titer lentiviral particles

Overview of the process for generating high-titer lentiviral particles. Through this proprietary, large-scale process Vectalys is able to produce very high-titer, high-purity lentiviruses ready for transduction of many different cell types. The high titer allows for manipulation of even hard-to-transduce cells, like primary cells or stem cells, and the exceptional purity prevents cytotoxicity.

Transduction efficiency of a Vectalys GFP-expressing lentiviral vector in IMR90 cells

Transduction efficiency of a Vectalys GFP-expressing lentiviral vector in IMR90 cells. Even with a low MOI of 5, >95 of cells are transduced using this high-titer lentivirus. Very high expression can be achieved by increasing the MOI.

Comparison of the purity of lentiviruses generated with Vectalys’ method or a classic purification method

Comparison of the purity of lentiviruses generated with Vectalys’ method or a classic purification method. Lentiviral proteins were separated by SDS-PAGE and stained with Coomassie Blue. The high purity of Vectalys lentiviral particles is clear when comparing the two methods; even when Vectalys particles are loaded at a 10-fold higher amount, very little contaminating protein is seen. The ratio of total particles (physical particles) to functional particles (transducing units) is lower for Vectalys purified lentivirus compared to classic methods of purification, indicating higher purity. Data provided by Vectalys.

Not all titers are the same! Comparison of cells transduced with 100 M

Not all titers are the same! Comparison of cells transduced with 100 M.O.I. of lentivirus provided by Clontech/Vectalys versus 100 M.O.I. of lentivirus provided by Vendor S. Each premade virus preparation expresses a green fluorescent protein when transduced. Clontech/Vectalys provides a functional titer, so every cell treated with our virus preparation was transduced with multiple copies of virus, leading to strong expression of the fluorescent protein. By contrast, the titer provided by Vendor S includes nonfunctional lentivirus particles as a large percentage of the titer so 100 M.O.I of their virus preparation is not sufficient to effectively transduce the cells.