AAV2 tetracycline-inducible expression system
The AAVpro Tet-One Inducible Expression System is a tetracycline-inducible gene expression system that allows you to produce high titers of recombinant, adeno-associated virus (AAV) for establishing a tightly inducible expression system for your gene of interest in mammalian cells. The all-in-one pAAV-TetOne vector expresses the Tet-On 3G transactivator from the constitutive human PGK promoter, and your gene of interest from the PTRE3GS promoter. In the presence of doxycycline (Dox), the Tet-On 3G transactivator specifically binds and activates high-level transcription from the inducible promoter that controls expression of your gene. There is no selection marker on this vector for mammalian cells. The kit also includes the plasmids for preparation of high-titer AAV particles without the use of a helper virus, and AAV Extraction Solution for isolation of pure AAV particles.
Overview
- All-in-one vector design allows you to establish tet-inducible expression of your gene of interest with a single vector
- Low background expression and high induction
- Transactivator expression from the human PGK promoter allows inducible expression in a broad range of tissues
- The unique AAV packaging system allows preparation of high-titer AAV2 without the use of a helper virus
- Kit includes AAV Extraction Solution that increases viral particle recovery as compared to conventional freeze-thaw methods
Applications
- Tightly controlled, tetracycline-inducible expression of your gene of interest in mammalian cells
