Terra PCR Direct Polymerase Mix

Brand	Takara Bio

A hot-start polymerase mix that is developed for direct PCR amplification from tissue samples, crude extracts, and dirty templates. It is ideal for amplifying short DNA targets (up to 2 kb), regardless of GC content or template purity. The polymerase mix is supplied with separate tubes of buffer (with Mg2+ and dNTPs) and Proteinase K. The inclusion of Proteinase K enhances gel detection of the PCR products.

Overview

Skip sample prep
Amplify directly from animal or plant tissues, or from crude extracts
Easily amplify GC-rich targets
Room-temperature setup with automatic hot start
Specialized kits including a convenient premix, an all-in-one kit that includes extraction buffer and loading dye, and FFPE and Whatman FTA sample processing formulations

Applications

Genotyping
Direct PCR from crude extracts, tissues, blood, FFPE samples and many more

Terra PCR Direct was used to amplify the cyclin D2 gene (Ccnd2, 0.5 kb; Lane 1) and the transferrin receptor gene (TfrC 2 kb; Lane 2) from 1 μl of mouse blood treated with either EDTA or heparin

Panel A. Terra PCR Direct was used to amplify the cyclin D2 gene (Ccnd2, 0.5 kb; Lane 1) and the transferrin receptor gene (TfrC, 2 kb; Lane 2) from 1 μl of mouse blood treated with either EDTA or heparin. Panel B. Terra PCR Direct was used to amplify the mouse Ywhaz1 gene (1 kb) directly from either a 1 mm tail or 1.5 mm2 ear biopsy. A 4 μl aliquot of each sample was mixed with gel loading buffer that either lacked or contained proteinase K (Lanes 1 and 2, respectively). The PCR products treated with proteinase K ran as expected, whereas those without proteinase K treatment got stuck in the wells. Panel C. Terra PCR Direct was used to amplify the cytochrome c oxidase gene (cox1; 0.5 kb) directly from 0.5 mm (Lane 1) and 1.2 mm (Lane 2) tomato or spinach leaf cuttings (made using hole punches).

Faster DNA amplification with the Terra PCR Direct FFPE Kit

Faster DNA amplification with the Terra PCR Direct FFPE Kit. The Terra FFPE Kit and a competitor’s kit were both used to amplify fragments of the GAPDH gene from FFPE rat leg. Lanes 1: 152 bp fragment. Lanes 2: 193 bp fragment. Lanes 3: 429 bp fragment. Lanes M: 100 bp ladder.

Terra PCR Direct Polymerase Mix

Additional information