Terra PCR Direct Genotyping Kit
This Genotyping Kit contains everything needed to genotype directly from animal tissue such as mouse tails. The Kit is based on the hot-start Terra PCR Direct DNA Polymerase Mix, a highly sensitive enzyme that allows amplification of targets from small amounts of template. In addition, the enzyme readily amplifies short DNA targets (up to 2 kb), regardless of GC content or template purity. Tissue Extract Buffer and PCR buffer (with Mg2+ and dNTPs) are included in the kit. A tube of Proteinase K is provided to enhance gel detection of the PCR products.
Overview
- Skip sample prep
- Amplify directly from animal or plant tissues, or from crude extracts
- Easily amplify GC-rich targets
- Room-temperature setup with automatic hot start
- Specialized kits including a convenient premix, an all-in-one kit that includes extraction buffer and loading dye, and FFPE and Whatman FTA sample processing formulations
Applications
- Genotyping
- Direct PCR from crude extracts, tissues, blood, FFPE samples and many more
