These products are powders for preparing Tris-Borate-EDTA (TBE) buffer used for agarose or polyacrylamide gel electrophoresis of nucleic acid.
Components
Tris-Borate-EDTA Buffer (TBE) 10x Powder, pH 8.3 (Cat. #T9122), 10 pouches (for 10 L)
Final solution from one pouch (1 L):
1X concentration
0.089 M Tris-Borate
0.002 M EDTA
Tris-Borate-EDTA Buffer (TBE) Powder, pH 8.3 (Cat. #T9121), 10 pouches (for 10 L)
Final solution from one pouch (1 L):
10X concentration
0.89 M Tris-Borate
0.02 M EDTA
TBE (Tris-Borate-EDTA) Powder (Cat. # T905), 30 packages (for 30 L)
One package (for 1 L) contains:
10.78 g Tris (hydroxymethyl) aminomethane
5.51 g Borate
0.75 g EDTA
Storage
Store at room temperature.
Note
TBE (Tris-Borate-EDTA) Powder (Cat. # T905) is manufactured by Roman Industries Co., Ltd.
Preparation
Transfer the contents of the pouch to a flask or beaker. Add 300 ml of distilled water or deionized water and stir the solution for a few minutes. Adjust the volume to 1,000 ml, and stir until completely dissolved.
