TB Green Advantage qPCR Premixes

Brand	Takara Bio
Size	200 Rxns

Brand

Size

200 Rxns

TB Green Advantage qPCR Premix is a convenient 2X master mix containing TB Green dye, full-length Taq DNA Polymerase, hot-start antibody, dNTPs, and buffer (including Mg2+). The mix comes with two separate tubes of ROX passive reference dye: ROX Reference Dye LSR contains the optimal concentration of ROX for instruments whose excitation source is a 488 nm laser; and ROX Reference Dye LMP contains the optimal concentration of ROX for instruments whose excitation source is either a lamp or an LED.

Overview

Provided in a convenient premixed format containing TB Green intercalating dye
Optimized for use with the laser-, lamp-, or LED-based real-time instrument of your choice
Compatible with all real-time PCR instruments

Applications

Real-time PCR/qPCR
Gene expression analysis
Genotyping
CpG island amplification

Compatible cyclers

SmartCycler, LightCycler, ABI PRISM 7000/7700/7900 HT, Applied Biosystems StepOne and StepOnePlus, 7300/7500, iCycler, Opticon, Stratagene MX 3000P and MX3005P, and QIAGEN Rotor-Gene.

Reaction specificity—performance of Takara Bio TB Green Advantage qPCR Premix vs. Competitor R’s qPCR mix using a Roche LightCycler.

Reaction specificity—performance of Takara Bio TB Green Advantage qPCR Premix vs. Competitor R’s mix using a Roche LightCycler. The results for the Takara Bio reagent are shown in Panels A and C, and those for Competitor R’s reagent are shown in Panels B and D. The cycling conditions for our reagent consisted of 1 cycle at 95°C for 10 min, followed by 45 cycles at 95°C for 5 sec and 60°C for 20 sec. For Competitor R’s reagent, the cycling conditions consisted of 1 cycle at 95°C for 10 min, followed by 45 cycles at 94°C for 10 sec, 55°C for 5 sec, and 72°C for 10 sec.

Amplification efficiency—performance of TB Green Advantage qPCR Premix vs. Competitor A’s qPCR mix using an ABI PRISM 7000 Sequence Detection System.

Amplification efficiency—performance of TB Green Advantage qPCR Premix vs. Competitor A’s SYBR mix using an ABI PRISM 7000 Sequence Detection System. The results for the TB Green reagent are shown in Panels A and C, and those for Competitor A’s reagent are shown in Panels B and D. The cycling conditions for the TB Green reagent consisted of 1 cycle at 95°C for 10 sec, followed by 40 cycles at 95°C for 5 sec and 60°C for 31 sec. For Competitor A’s reagent, the cycling conditions consisted of 1 cycle at 95°C for 10 min, followed by 45 cycles at 95°C for 15 sec and 60°C for 1 min.

Reaction specificity—Takara Bio’s TB Green Advantage qPCR Premix vs. Competitor I’s qPCR mix using a Cepheid Smart Cycler.

Reaction specificity—performance of Takara Bio’s TB Green Advantage qPCR Premix vs. Competitor I’s qPCR mix using a Cepheid Smart Cycler. The results for the Takara Bio reagent are shown in Panels A and C, and those for Competitor I’s reagent are shown in Panels B and D. The cycling conditions for the TB Green reagent consisted of 1 cycle at 95°C for 2 min, followed by 45 cycles at 95°C for 5 sec and 60°C for 20 sec. For Competitor I’s reagent, the cycling conditions consisted of 1 cycle at 95°C for 2 min, followed by 45 cycles at 95°C for 15 sec and 60°C for 30 sec.

TB Green Advantage qPCR Premixes

Additional information