TaqI (TthHB8I)

Additional information

Brand

TaqI (TthHB8I) restriction site:

T | CG A
A GC | T

Supplied buffer: basal

Effect of DNA methylation: enzyme activity is affected by dam methylase

Reaction temperature: 65°C

  • Source: Escherichia coli carrying the plasmid encoding TaqI gene
  • Concentration: 4–12 units/µl
  • Substrate for unit definition: lambda DNA
  • Star activity: cleavage at alternative site(s) occurs in the presence of high concentrations of glycerol, Mn2+, DMSO, or at low ionic strength
  • Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning