TaKaRa Taq DNA Polymerase and premix
TaKaRa Taq DNA Polymerase is a recombinant version Taq polymerase derived from the Thermus aquaticus YT-1 strain, and is suitable for routine PCR applications.
For individual reaction setup and optimization, use individual components of enzyme, dNTP mixture, and 10X reaction buffer (with or without Mg2+). A convenient 2X PCR master mix of Takara Taq enzyme, buffer, and dNTP mixture is available as Premix Taq DNA Polymerase (TaKaRa Taq Version 2.0) (Cat. # R004A).
Two other formulations of TaKaRa Taq DNA polymerase are also available:
- Several antibody-mediated hot-start versions of Takara Taq—for preventing non-specific amplification from mispriming during reaction setup
- GMP-grade version—for an added level of confidence in our quality polymerase
Overview
- Excellent for standard PCR amplifications
- Low bacterial DNA contamination
- Premix Taq enables easy PCR setup and minimizes pipetting steps
Applications
- Routine PCR
- DNA sequencing
Source
- Recombinant E. coli
PCR products
The resulting PCR product can be cloned directly into a T-vector (TA cloning) due to the addition of an adenosine (A) to the 3′ end. It is also possible to clone the PCR product into blunt-end vectors after blunting and phosphorylation of the ends.
