The Pluripotency Check PCR Kit contains all the necessary reagents to confirm that your mouse embryonic stem (ES) or induced pluripotent (iPS) cells are indeed pluripotent. It includes 16 prevalidated forward and reverse primer mixes, based on nine genes expressed in pluripotent stem cells and two control genes. The different amplicon sizes allow you to distinguish between endogenous and exogenous expression. The kit includes everything for start-to-finish confirmation of your cells’ pluripotency status, using any combination of primer sets, or the entire panel.
Overview
- Validate the pluripotent status of your ES or iPS cell culture using a simple PCR kit
- 16 prevalidated PCR primer mixes
- Complete kit with RNA extraction, reverse transcription, and PCR reagents
- Confirm your iPS cells by differentiating between endogenous and exogenous gene expression
Applications
- Validating mouse embryonic stem cell status
- Confirming iPS status
