SphI

Brand	Takara Bio

SphI restriction site:

G CATG | C
C | GTAC G

Supplied buffer: H

Effect of DNA methylation: enzyme activity is not affected by CG methylase

Reaction temperature: 37°C

Source: Streptomyces phaeochromogenes
Concentration: 4–12 units/µl
Substrate for unit definition: lambda DNA
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning