SMARTer universal low input RNA kit—cDNA synthesis for NGS from degraded samples
The SMARTer Universal Low Input RNA Kit for Sequencing provides a simple and efficient solution for generating cDNA from very low amounts of input RNA that is compromised, has a low RIN value, and/or lacks a poly(A) tail. The kit was specifically designed for RNA-Seq applications involving FFPE or laser-captured samples, and works with as little as 200 pg and up to 10 ng of rRNA-depleted material. It outperforms existing methods in terms of gene-body coverage, data reproducibility, read mappability, and ERCC correlation. For upstream rRNA removal from 10-100 ng of input total RNA, we recommend the RiboGone – Mammalian kit.
cDNA produced with this kit is compatible with low-input NGS library preparation kits, including those for Illumina and Ion Torrent.
Overview
- Sensitivity for analysis of low-input samples—works with 200 pg to 10 ng of rRNA-depleted input (derived from 2–100 ng of total RNA)
- Efficient results from compromised samples—an ideal solution for FFPE samples
- High quality RNA-seq data—consistent RNA-seq data across the recommended input range (200 pg–10 ng)
- Mappability >80%
- Reproducibility >0.98
- ERCC correlation >0.99
- Number of genes >15,000
- Kit includes both RT and PCR enzymes
Applications
- Generate cDNA for RNA-seq from very low amounts of RNA (200 pg–10 ng) that:
- Is compromised (e.g., obtained from FFPE or LCM samples)
- Has low RIN values (<8)
- Is not polyadenylated
