The SMARTer ThruPLEX Plasma-Seq Kit builds on the innovative ThruPLEX chemistry to generate high-complexity DNA libraries from cell-free DNA isolated from plasma. Single index, dual index, and unique dual index kits are available and must be purchased separately.
Features
- Designed for cell-free DNA—newly formulated repair and ligation reagents with optimized protocols
- High-performance NGS libraries—high library diversity with broad and reproducible GC coverage
- Variable sample input—<1 ng to 30 ng of cell-free DNA
- Fast and simple workflow—three steps in a single tube or well in two hours, with no purification or sample transfer steps (tubes and plates sold separately)
- Research applications—construct libraries from samples where results require high sensitivity, including liquid biopsies, ctDNA, and targeted sequencing
- Automation friendly—compatible with the Beckman FXP Workstation
Guelfi, G. et al. Next generation sequencing of urine exfoliated cells: an approach of prostate cancer microRNAs research. Sci. Rep. 8, 7111 (2018).
Mayrhofer, M. et al. Cell-free DNA profiling of metastatic prostate cancer reveals microsatellite instability, structural rearrangements and clonal hematopoiesis. Genome Med. 10, 85–98 (2018).
Mouliere, F. et al. Detection of cell‐free DNA fragmentation and copy number alterations in cerebrospinal fluid from glioma patients. EMBO 12, e9323 (2018).
Murtaza, M. et al. Non-invasive analysis of acquired resistance to cancer therapy by sequencing of plasma DNA. Nature 497, 108–112 (2013).
Patel, K. M. et al. Association of plasma and urinary mutant DNA with clinical outcomes in muscle invasive bladder cancer. Sci. Rep. 7, 5554 (2017).
Xia, Y. et al. Copy number variations in urine cell free DNA as biomarkers in advanced prostate cancer. Oncotarget 7, 35818–35831 (2016).
