SfiI

Brand	Takara Bio

SfiI restriction site:

GGCCN NNN | NGGCC
CCGGN | NNN NCCGG

Supplied buffer: M

Effect of DNA methylation: enzyme activity is often affected by dcm methylase

Reaction temperature: 50°C

Source: Streptomyces fimbriatus
Concentration: 4–12 units/µl
Substrate for unit definition: Ad2 DNA
Genome DNA analysis: Escherichia coli genomic DNA
Star activity: cleavage at alternative site(s) occurs in the presence of Mn2+ or DMSO
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning