SeqAmp DNA Polymerase

Brand	Takara Bio

SeqAmp DNA Polymerase is a high-fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit.

SeqAmp™ CB PCR Buffer is designed for improved compatibility with DNA purification using Agencourt AMPure beads after amplification with SeqAmp DNA Polymerase and works with SMARTer® next-generation sequencing kits.

Overview

Recommended for use with SMARTer next-generation sequencing kits.

Applications

High-fidelity PCR enzyme sold as part of several SMARTer kits for next-generation sequencing, used to amplify purified first-strand cDNA into RNA-seq libraries.
The SeqAmp CB PCR Buffer (Cat. # 638526) is an optional buffer that may substitute for the SeqAmp PCR Buffer for easier bead resuspension when using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing. Note that for the SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian, the SeqAmp CB PCR Buffer is included as part of the kit.

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit).

SeqAmp CB PCR buffer improves bead-pellet formation

Improved bead-pellet formation with new SeqAmp CB PCR buffer. The PCR buffer included in the Pico v2 kit was re-formulated to allow for faster, tighter bead-pellet formation. Following magnetic separation for a fixed period, bead pellets formed in the new SeqAmp CB buffer (right) are tighter than those formed in the original PCR buffer (left). Tighter bead pellets tend to dry more evenly and are easier to resuspend than pellets that are broader and more diffuse.

SeqAmp DNA Polymerase

Additional information