Sau3AI (MboI)

Brand	Takara Bio

Sau3AI (MboI) restriction site:

| GATC
CTAG |

Supplied buffer: H

Effect of DNA methylation: enzyme activity is affected by CG methylase

Reaction temperature: 37°C

Source: Staphylococcus aureus 3A
Concentration: 4–12 units/µl
Substrate for unit definition: lambda DNA
Star activity: Cleavage at alternative site(s) occurs the presence of DMSO or high concentrations of glycerol. Known alternative recognition sites include: SATC, GMTC, GAKC, and GATS (where S represents G or C; M represents A or C; and K represents G or T).
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning