SAN High Quality (Bioprocessing grade) is the ultimate solution for efficient removal of nucleic acids in manufacturing and bioprocessing workflows. This nonspecific, recombinant endonuclease has optimum activity at high salt concentrations, which can improve efficiency and yield in various workflows.
The Main Advantages of SAN High Quality
- High purity (≥ 98%)
- No protease detected
- Animal origin-free production
- Endotoxin-tested
- Active at low temperatures
- High activity at high salt conditions
- Supplied with extended product documentation
- Compatible with SAN HQ ELISA
Properties
- Source: Recombinantly produced in Pichia pastoris
- Specific activity: ≥ 175 000 Units/mg
- Size: The protein is glycosylated. Protein size without glycosylation is 26 kDa
- Unit definition: One Unit is defined as an increase in absorbance at 260 nm of 1 A in 30 minutes at 37°C, using 50 μg/ml calf thymus DNA in a buffer consisting of 25 mM Tris-HCl, pH 8.5 (25°C), 5 mM MgCl2, 500 mM NaCl
- Specificity: Nonspecific endonuclease cleaving single- and double stranded DNA and RNA. The enzyme degrades DNA vs. RNA in a 10:1 ratio
- End-product: Following complete degradation, the end product consist of a majority of 5 nucleotide oligos
- Activity:
- Tmp: 0–40°C, optimal 35°C
- NaCl: 50mM–1M, optimal NaCl is 0.5 M
- Mg2+ (>1 mM) is required for activity, optimal Mg2+ is 20 mM
- pH: 7.0–9.5, optimal pH is 9
- Tolerance to typical buffer additives:
- Imidazole: 20% activity at 350 mM Imidazole
- Glycerol: 20% activity at 35% glycerol
- Triton X-100: No reduction in activity (tested up to 15%)
- SDS: Not recommended
- Urea: Not recommended
- Reducing agents (e.g. DTT, TCEP): will result in inactivation
SAN HQ ELISA Kit
ArcticZymes offers SAN HQ ELISA Kit to confirm the removal of SAN High Quality (Bioprocessing Grade) in bioprocessing and biomanufacturing applications.
