SalI

Brand	Takara Bio

SalI restriction site:

G | TCGA C
C AGCT | G

Supplied buffer: H

Effect of DNA methylation: enzyme activity is affected by CG methylase

Reaction temperature: 37°C

Source: Streptomyces albus G
Concentration: 8–20 units/µl [High concentration: 30–60 units/µl]
Substrate for unit definition: lambda DNA
Genomic DNA analysis: Staphylococcus aureus genomic DNA
Star activity: cleavage at alternative site(s) occurs in the presence of DMSO or high concentrations of glycerol
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning