SacII

Brand	Takara Bio

SacII restriction site:

CC GC | GG
GG | CG CC

Supplied buffer: T+BSA

Effect of DNA methylation: enzyme activity is affected by CG methylase

Reaction temperature: 37°C

Source: Streptomyces achromogenes
Concentration: 8–20 units/µl
Substrate for unit definition: Ad2 DNA
Genome DNA analysis: Staphylococcus aureus genomic DNA
This enzyme is able to cleave at 4 lambda DNA sites, but the site at position 40386 is resistant to cleavage
Each lot undergoes stringent quality testing, including: overdigestion, genome DNA analysis, ligation-recutting, and pKF3 Cloning