SacI

Brand	Takara Bio

SacI restriction site:

G AGCT | C
C | TCGA G

Supplied buffer: L

Effect of DNA methylation: enzyme activity is not affected by CG methylase

Reaction temperature: 37°C

Source: Streptomyces achromogenes
Concentration: 4–12 units/µl [High concentration: 30–60 units/µl]
Substrate for unit definition: lambda DNA
Star activity: cleavage at alternative site(s) occurs in the presence of DMSO or high concentrations of glycerol
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning