RHB-A, supplemented with Epidermal Growth Factor (EGF) and Fibroblast Growth Factor-2 (FGF-2), enables the maintenance and continual expansion of symmetrically-dividing NS cells in defined, serum-free adherent culture. In growth factor supplemented RHB-A, NS cells have been demonstrated to retain their neurogenic capacity for over 100 generations, with full maintenance of diploid karyotype. RHB-A in the presence of EGF and FGF-2 also supports the derivation of clonogenic NS cell lines. Culture of adherent NS cells in RHB-A with sequential growth factor withdrawal leads to differentiation into functional neurons. RHB-A supplemented with EGF and FGF-2 has recently been used for the propagation of glioblastoma stem cell lines.
RHB-Basal is a proprietary, defined, serum-free basal medium specifically formulated for the propagation and differentiation of adherent NS cells. RHB-Basal does not contain any growth factors or neuronal supplements. Therefore, customers can tailor RHB-Basal to suit the specific requirements of their cell type by the addition of preferred supplements.
Overview
- RHB-A is a proprietary, fully defined, and serum-free medium designed to maintain pure populations of adherent human and mouse NS cells
- RHB-Basal medium is free of animal components and neuronal supplements; the medium can be customized by the addition of supplements
Applications
- Derivation of mouse and human NS cells from ES cells and fetal and adult tissues
- Maintenance and propagation of adherent mouse and human NS cells
- Differentiation of mouse and human NS cells into functional neurons
- Differentiation of mouse ES cells to neuronal precursors
- Organotypic slice culture of CNS tissue
- Glioblastoma stem cell culture for cancer research
- Refer to the Data Sheet for additional examples of use
