An NIH 3T3-derived cell line designed for the production of infectious, replication-incompetent virus. PT67 contains the Moloney murine leukemia virus (MoMuLV) gag, pol, and env (10A1-derived) genes. Transfection with a retroviral vector containing the retroviral packaging signal and a target gene allows production of replication-incompetent virus.
Overview
- We recommend the Retro-X Universal Packaging System for most applications
- This system produces the highest titers
- GP2-293 cells are easy to transfect which leads to very high titers
- It is supplied with all four commonly used envelopes for great flexibility (including VSV-G and Eco)
- Retrovirus is produced by transient transfection of the envelope vector and retrovirus vector
- Use RetroPack cells if you wish to create a stable producer cell line
- All retroviral packaging cell lines and systems are compatible with all retroviral vectors sold by Takara Bio, including all MMLV- and MSCV-based retroviruses.
