This kit provides a complete retroviral vector packaging system for use with any retroviral vector. It is designed to produce virus that can infect dividing cells from a broad range of mammalian and nonmammalian cell types. The GP2-293 Packaging Cell Line contains only the MoMuLV gag and pol genes. The viral envelope portion of the packaging function (env gene) is supplied by transiently co-transfecting one of the Retro-X Universal Packaging Vectors (p10A1, pAmpho, pEco, or pVSV-G) with the retroviral vector. The pQCLIN Retroviral Vector (included in the vector set) can be used as a positive control to constitutively express β-galactosidase in transduced mammalian cells.
Overview
- We recommend the Retro-X Universal Packaging System for most applications
- This system produces the highest titers
- GP2-293 cells are easy to transfect which leads to very high titers
- It is supplied with all four commonly used envelopes for great flexibility (including VSV-G and Eco)
- Retrovirus is produced by transient transfection of the envelope vector and retrovirus vector
- Use RetroPack cells if you wish to create a stable producer cell line
- All retroviral packaging cell lines and systems are compatible with all retroviral vectors sold by Takara Bio, including all MMLV- and MSCV-based retroviruses.
