Retro-X Tet-On 3G Inducible Expression System

The Tet-On 3G systems allow inducible gene expression only in the presence of doxycycline (Dox)

The Tet-On 3G systems allow inducible gene expression only in the presence of doxycycline (Dox). When Dox binds, the transactivator undergoes a conformational change allowing it to bind tet operator (tetO) repeats within the TRE3G promoter.

Vector formats for lentiviral (pLVX) and retroviral (pRetroX) Tet-On 3G tetracycline inducible expression systems

Vector formats for lentiviral (pLVX) and retroviral (pRetroX) Tet-On 3G tetracycline inducible expression systems. Each system includes a regulator vector that stably expresses the Tet-On 3G transactivator protein, and a response vector that contains the PTRE3GV promoter that controls the expression of your transgene.

Tetracycline-inducible expression combined with retroviral delivery

Tetracycline-inducible expression combined with retroviral delivery. MCF7 cells were co-transduced at a 1:1 ratio with Retro-X Tet-On 3G retrovirus and Retro-X-TRE3G-Luc retrovirus engineered for inducible luciferase expression. Transduced cells were grown in the absence or presence of Dox for 48 hr and assayed for luciferase activity. RLU = relative light units.

Inducible expression of luciferase using transduced MMLV retrovirus

Inducible expression of luciferase using transduced MMLV retrovirus. VSV-G pseudotyped RetroX-Tet3G and RetroX-TRE3G-Luc retrovirus were generated with the Retro-X Universal Packaging System (supplied) and used to co-infect HepG2 cells at a 1:1 ratio. After 24 hr, the transduced cells were treated with the indicated dose of doxycycline (Dox). Expression of luciferase was determined a further 48 hr later using an anti-luciferase antibody on a Western blot.