The Ready-To-Glow Dual Secreted Reporter Assay contains the buffers and substrates for use with the vectors contained in the Ready-To-Glow Dual Secreted Reporter Vector Kit (Cat. No. 631735). This assay allows you to monitor the activity of two promoters simultaneously. The promoters are monitored by testing the media from cells that have been transfected with the two reporter vectors, which express luciferase and SEAP respectively. The assay allows you to monitor promoter activity over time, because samples of the cell media can be taken repeatedly and tested without lysing cells.
Overview
- Secreted luciferase assay protocol eliminates cell lysis
- 2–4 fold higher signal than firefly or Renilla luciferase
- Single transfection allows multiple time points from same cell
- Dual, live-cell assay includes SEAP as well as secreted luciferase:
monitor two promoters, or use one reporter as a normalization control
Applications
- Homogeneous assay protocols
- Time-course studies
- Test multiple compounds
- Suitable for downstream experiments with the same cells
- Use the dual assay (which includes secreted alkaline phosphatase, as well as a secreted luciferase assay) to monitor two promoters
- Use the dual assay to monitor one promoter with a built-in normalization control
