Random primers
Random Primers are 6-mer and 9-mer deoxyribonucleotide mixtures composed of a random sequence (up to 46 or 49 different sequences, respectively) and a phosphorylated 5′-end. Both the pd(N)6 Random Primer and the pd(N)9 Random Primer contain approximately 150 µg (5 OD) and are suitable for cDNA synthesis and hybridization applications.
Applications
- Single-tube cDNA synthesis
- Hybridization experiments using labeled probe (120 pmol primer/µg DNA recommended)
- First-strand cDNA synthesis during RT-PCR
