Set of three vectors for studying Raf or MAPK signaling pathways: the pCMV-Raf-1 Vector expresses the wild-type Raf protein, the pCMV-RafCAAX expresses a constitutively active form of the Raf protein, and the pCMV-RafS621A Vector expresses a mutated form of Ras with a Ser-to-Ala mutation at residue 621. These vectors can be used to study Raf-related signal transduction pathways in mammalian cells. Each gene is expressed from the constitutive CMV promoter.
Overview
- Simplify the complexities of signal transduction in vivo
- Dissect the network of signal transduction pathways
- Screen for compounds that activate key pathways
- Combine with cis reporter vectors to create a complete assay system
