Reference RNA for real-time qPCR
A mixture of total RNAs from a collection of adult human tissues, chosen to represent a broad range of expressed genes. Both male and female donors are represented.
qPCR Reference Total RNA serves as a standard for the accurate and reproducible comparison of gene expression data using real-time quantitative PCR (qPCR). It may also be used as a source of positive control templates for validating qPCR primer designs.
Total RNA was isolated by a modified guanidinium thiocyanate method.
Overview
- Featuring the broadest possible gene representation with minimal lot-to-lot variation
- Use with any microarray or labeling method
- Less gene-to-gene signal variation
- Virtually free of genomic DNA
Applications
- Microarray analysis
- Real-time PCR/qPCR
- RT-PCR
- Northern analysis
- Gene-expression analysis
