Reference cDNA for real-time qPCR
cDNAs prepared from a mixture of total RNAs collected from adult normal human tissues. This cDNA mixture has been chosen to represent a broad range of expressed genes.
qPCR Human Reference cDNA serves as a standard for accurate and reproducible comparison of gene expression data using qPCR (quantitative PCR) as well as a positive control template for validation of gene primer design. Enough cDNA is supplied for either 25 or 100 reactions requiring 2 μl of sample per reaction; the number of reactions is based on a 50 μl reaction volume.
The sample was oligo(dT) primed.
Overview
- Broad gene coverage
- Virtually free of genomic DNA
- Made from human tissues, not cultured cell lines
- A high-performance standard for quantitative PCR
Applications
- Real-time PCR/qPCR
- Gene expression analysis
- One-step RT-PCR
- Northern analysis
- Microarray analysis
