pZsYellow1-N1 is a human codon-optimized expression vector that encodes a variant of the Zoanthus sp. yellow fluorescent protein, ZsYellow1, which has been engineered for brighter fluorescence and higher expression in mammalian cells. pZsYellow1-N1 allows genes cloned into the multiple cloning site (MCS) upstream of the ZsYellow1 coding sequence to be expressed as fusions to the N-terminus of the fluorescent protein. The unmodified vector will express ZsYellow1 in mammalian cells.
Overview
- Bright yellow fluorescent protein
- ZsYellow1 excitation and emission maxima are 529 and 539 nm, respectively
Applications
- Fusions
- Protein localization
- General reporter for mammalian cells
- Monitoring transfection efficiencies
