PvuII

Brand	Takara Bio

PvuII restriction site:

CAG | CTG
GTC | GAC

Supplied buffer: M

Reaction temperature: 37°C

Source: Proteus vulgaris
Concentration: 8–20 units/µl [High concentration: 30–60 units/µl]
Substrate for unit definition: lambda DNA
Star activity: Cleavage at alternative sequences occurs in the presence of DMSO or high concentrations of glycerol. Known alternative cleavage sites include: NAGCTG, CNGCTG, CANCTG, CAGNTG, CAGCNG, and CAGCTN
Each lot undergoes stringent quality testing, including overdigestion, genomic DNA analysis, ligation-recutting, and pKF3 cloning