PvuI

Brand	Takara Bio

PvuI restriction site:

CG AT | CG
GC | TA GC

Supplied buffer: K + BSA

Effect of DNA methylation: Enzyme activity is affected by CG methylase, but not dam methylase

Reaction temperature: 37°C

Source: Escherichia coli carrying the plasmid encoding the Pvu I gene
Concentration: 4–12 units/µl
Substrate for unit definition: lambda DNA
Genome DNA analysis: Flavobacterium okeanokoites genome DNA
Ligation-recutting test: The ligation efficiency of cohesive-ended DNA fragments generated by this enzyme will be low. More efficient ligation can be achieved using blunt end ligation reaction conditions
Each lot undergoes stringent quality testing, including overdigestion, genomic DNA analysis, ligation-recutting, and pKF3 cloning