PstI

Brand	Takara Bio

PstI restriction site:

C TGCA | G
G | ACGT C

Supplied buffer: H

Reaction temperature: 37°C

Source: Escherichia coli ED8654 carrying the plasmid encoding Pst I gene
Concentration: 8–20 units/µl [High concentration: 30–60 units/µl]
Substrate for unit definition: lambda DNA
Star activity: cleavage at alternative site(s) occurs in the presence of DMSO or high concentrations of glycerol
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning