PshAI

Brand	Takara Bio

PshAI restriction site:

GACNN | NNGTC
CTGNN | NNCAG

Supplied buffer: K

Effect of DNA methylation: enzyme activity is affected by CG methylase

Reaction temperature: 37°C

Source: Plesiomonas shigelloides
Concentration: 10 units/µl
Substrate for unit definition: lambda DNA
Do not dilute. This enzyme is unstable at low concentration. Addition of BSA to a final concentration of 0.01 in the reaction mixture increases its relative activity to 140 at 37°C and 220 at 30°C.
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning