The Proteasome Sensor Vector (pZsProSensor-1) encodes the gene for Zoanthus sp. Green Fluorescent Protein (ZsGreen) fused to the mouse ornithine decarboxylase (MODC) degradation domain (amino acids 410–461). This vector is designed for studies of proteasome function in mammalian cells: Since the MODC degradation domain targets the constitutively-expressed protein for rapid degradation, the protein does not accumulate in cells until the proteasome is inhibited, which is indicated by an increase in green fluorescence. This vector also contains a kanamycin/neomycin selectable marker, as well as SV40 and pUC origins of replication.
Overview
- Easy fluorescent monitor of proteasome activity
- Compatible with individual cells or whole populations in real time
- Easy, non-invasive detection
- Simple and cost effective assay
- High-throughput compatible
Applications
- Drug screens
- Cancer studies
- Image-based HTS/HCS
- Real-time proteasome studies
