PrimeSTAR Max DNA Polymerase

Brand	Takara Bio

A 2X hot-start PCR master mix that is ideal for high-fildelity PCR or high-throughput applications. PrimeSTAR Max DNA polymerase pairs Takara Bio’s high-fidelity PrimeSTAR HS DNA Polymerase with an elongation factor to provide efficient priming and extension, thereby shortening annealing and extension times. Please refer to the User Manual for the master mix composition.

Overview

Highest fidelity of any commercially available DNA polymerase
Fastest extension speed means less time required for PCR cycles
Convenient 2X premix includes buffer and dNTPs, allowing reaction assembly in less time
Antibody-mediated hot-start formulation
Proven performance as reported in peer-reviewed literature

Applications

Fast PCR for high-throughput studies
Cloning and expression studies
Structure/function studies
Evolutionary studies (e.g., SNP analyses)

PCR product termini

PrimeSTAR Max DNA Polymerase produces products with blunt ends. It is suitable for use in conjunction with blunt-ended cloning systems.

Recommended maximum amplicon length

Up to 6 kb (human genomic DNA template or human cDNA template)
Up to 10 kb (E. coli genomic DNA template)
Up to 15 kb (lambda DNA template)

The mutation frequency of PrimeSTAR Max DNA Polymerase and four other DNA Polymerases was examined by analysis of sequencing data

The mutation frequency of PrimeSTAR Max DNA Polymerase and four other DNA Polymerases was examined by analysis of sequencing data. Eight arbitrarily selected GC-rich regions were amplified with PrimeSTAR Max DNA Polymerase or other DNA polymerases, using Thermus thermophilus HB8 genomic DNA as template. PCR products (~500 bp each) were each cloned into a suitable plasmid. Multiple clones were selected per respective amplification product and were subjected to sequence analysis. Sequence analysis of DNA fragments amplified using PrimeSTAR Max DNA Polymerase demonstrated only 9 mismatched bases per 230,129 total bases. This is higher fidelity than an alternative high-fidelity enzyme from Company A, and 10-fold higher fidelity than Taq DNA polymerase.

PrimeSTAR Max DNA Polymerase

Additional information