This kit for two-step RT-PCR is designed to provide excellent extendibility and efficient amplification. It uses PrimeScript reverse transcriptase and Takara Ex Taq HS polymerase. This kit generates RT-PCR products efficiently at standard reverse transcription temperature (42℃), even when RNA templates contain higher-order structures. It is not necessary to perform reactions at a high temperature when there is a risk of RNA decomposition. This kit includes all reagents necessary for the reverse transcription of RNA to cDNA, and for cDNA amplification using PCR.
Overview
- RT-PCR kit includes all reagents needed for two-step endpoint RT-PCR (e.g., where products are analyzed using gel electrophoresis)
- Robust reverse transcription and amplification even from challenging RNA templates
- Complete elongation of complex RNA templates
- High-yield transcription of full-length cDNA up to 12 kb in length
- Minimal false priming and robust amplification due to the combination of PrimeScript RT with Takara Ex Taq HS
Applications
- Two-step RT-PCR
