PrimerArray Series for Biological Pathways

Procedural overview for gene expression analysis by real-time RT-PCR

The overall workflow for the PrimerArray Series primer sets is straightforward:

1. RNA extraction (30 min): NucleoSpin RNA (Cat. # 740955.50, not available in all geographic locations) is recommended.
2. Reverse transcription (20 min): PrimeScript RT Master Mix (Perfect Real Time) (Cat. # RR036A) is recommended.
3. Dispense real-time PCR reaction solution (10 min): Prepare a master mix containing the cDNA sample and TB Green Premix Ex Taq II (Tli RNase H Plus) (Cat.# RR820A); dispense into a 96-well plate.
4. Add PrimerArray primers (5 min): Use 4 µl of PrimerArray Series primers per reaction
5. Real-time PCR (1–2 hours): PrimerArray Series primers are compatible with all commonly used real-time PCR systems
6. Data analysis (30 minutes): Use the PrimerArray Analysis Tool

PrimerArray Analysis Tool

The PrimerArray Analysis Tool facilitates data analysis, and can be downloaded here. To access an instruction guide about how to use the tool, click on the Details down arrow icon in the product table, click on the Documents tab, and then expand the drop-down menu under User Manuals.

Available PrimerArray Primer Sets

Human

• PH001 – Cytokine-cytokine receptor interaction
• PH002 – Cell cycle
• PH003 – Cell adhesion molecules (CAMs)
• PH004 – Jak-STAT signaling pathway
• PH005 – Natural killer cell mediated cytotoxicity
• PH006 – Axon guidance
• PH007 – Focal adhesion
• PH009 – TGF-beta signaling pathway
• PH010 – Wnt signaling pathway

Mouse

• PN001 – Cytokine-cytokine receptor interaction
• PN002 – Cell cycle
• PN003 – Cell adhesion molecules
• PN004 – Jak-STAT signaling pathway
• PN005 – Natural killer cell mediated cytotoxicity
• PN006 – Axon guidance
• PN007 – Focal adhesion
• PN008 – T-cell receptor signaling pathway
• PN009 – TGF-beta signaling pathway
• PN010 – Wnt signaling pathway