PNGase F (Glycopeptidase F) removes N-glycans from glycoproteins and enables proteomic analysis, including MALDI-TOF mass spectrometry.
Applications
- Determination of protein and/or peptide glycosylation level
- Analysis of N-glycan functional roles
- Reduction of protein heterogeneity
- MALDI-TOF or other mass spectrometry
Components
- Denaturing buffer: 1% SDS / 1M Tris-HCl (pH 8.6; 500 μl)
- Native buffer: 1M Tris-HCl (pH 8.6; 500 μl)
- Stabilized solution: 5% NP-40 (500 μl)
- Control glycoprotein: 10 mg/ml Bovine Fetuin (10 μl)
Source
Recombinant Escherichia coli encoding PNGase F.
